Pflügers Archiv

, Volume 411, Issue 3, pp 283–288

Changes in intracellular ion activities induced by adrenaline in human and rat skeletal muscle

  • Klaus Ballanyi
  • Peter Grafe
Excitable Tissues and Central Nervous Physiology

DOI: 10.1007/BF00585116

Cite this article as:
Ballanyi, K. & Grafe, P. Pflugers Arch. (1988) 411: 283. doi:10.1007/BF00585116

Abstract

To study the stimulating effect of adrenaline (ADR) on active Na+/K+ transport we used double-barrelled ion-sensitive micro-electrodes to measure the activities of extracellular K+ (aKe) and intracellular Na+ (aNai) in isolated preparations of rat soleus muscle, normal human intercostal muscle and one case of hyperkalemic periodic paralysis (h.p.p.). In these preparations bath-application of ADR (10−6 M) resulted in a membrane hyperpolarization and transient decreasesaKe andaNai which could be blocked by ouabain (3×10−4 M). In the h.p.p. muslce a continuous rise ofaNai induced by elevation ofaKe to 5.2 mM could be stopped by ADR. In addition, the intracellular K+ activity (aKi), the free intracellular Ca2+ concentration (pCai) and intracellular pH (pHi) were monitored in rat soleus muscle. During ADRaKi increased, pHi remained constant and intracellular Ca2+ apparently decreased. In conclusion, our data show that ADR primarily stimulates the Na+/K+ pump in mammalian skeletal muscle. This stimulating action is not impaired in the h.p.p. muscle.

Key words

Ion activitiesNa+/K+ pumpAdrenalineSkeletal muscleHyperkalemic periodic paralysis

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Klaus Ballanyi
    • 1
  • Peter Grafe
    • 2
  1. 1.Institut für Zoologie IUniversität DüsseldorfDüsseldorf 1Germany
  2. 2.Physiologisches Institut der Universität MünchenMünchen 2Germany