Chromosoma

, Volume 101, Issue 7, pp 448–455

The genome of the Mediterranean fruitflyceratitis capitata: Localization of molecular markers by in situ hybridization to salivary gland polytene chromosomes

  • A. Zacharopoulou
  • M. Frisardi
  • C. Savakis
  • A. S. Robinson
  • P. Tolias
  • M. Konsolaki
  • K. Komitopoulou
  • F. C. Kafatos
Original Articles

DOI: 10.1007/BF00582839

Cite this article as:
Zacharopoulou, A., Frisardi, M., Savakis, C. et al. Chromosoma (1992) 101: 448. doi:10.1007/BF00582839

Abstract

We hybridized cloned DNA segments to salivary gland polytene chromosomes of the medfly,Ceratitis capitata, and thus established molecular markers for 24 sites on 6 out of 10 autosomal arms. An additional marker identified a medfly repetitive element that hybridizes to approximately 100 autosomal sites as well as a granular network that is thought to represent theX chromosome. Some of the markers correspond to 9 characterized transcription units, while 17 remain anonymous; at least 3 of the latter are restriction fragment length polymorphism (RFLP) markers. The characterized transcription units document that chromosomal arm 5L ofC. capitata is homologous to theDrosophila melanogaster X chromosome, in agreement with previous inferences based on the extensive conservation of linkage groups in Diptera.

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • A. Zacharopoulou
    • 1
  • M. Frisardi
    • 2
  • C. Savakis
    • 3
    • 4
  • A. S. Robinson
    • 3
  • P. Tolias
    • 2
  • M. Konsolaki
    • 3
  • K. Komitopoulou
    • 5
  • F. C. Kafatos
    • 2
    • 3
  1. 1.Department of Biology, Division of Genetics, Cell and Developmental BiologyUniversity of PatrasGR-PatrasGreece
  2. 2.Department of Cellular and Development BiologyHarvard UniversityCambridgeUSA
  3. 3.Institute of Molecular Biology and Biotechnology-FORTHHeraklion, Crete, Greece
  4. 4.Department of Medical SciencesUniversity of Crete Medical SchoolGR-Heraklion, Crete, Greece
  5. 5.Department of Biochemistry, Cell and Molecular Biology and GeneticsUniversity of AthensAthensGreece

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