Pflügers Archiv

, Volume 412, Issue 3, pp 297–304

Adenosine-5′-triphosphate-sensitive ion channels in neonatal rat cultured central neurones

Authors

  • M. L. J. Ashford
    • Department of PharmacologyUniversity of Cambridge
  • N. C. Sturgess
    • Department of PharmacologyUniversity of Cambridge
  • N. J. Trout
    • Department of PharmacologyUniversity of Cambridge
  • N. J. Gardner
    • Department of Clinical BiochemistryNew Addenbrooke's Hospital
  • C. N. Hales
    • Department of Clinical BiochemistryNew Addenbrooke's Hospital
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

DOI: 10.1007/BF00582512

Cite this article as:
Ashford, M.L.J., Sturgess, N.C., Trout, N.J. et al. Pflugers Arch. (1988) 412: 297. doi:10.1007/BF00582512

Abstract

ATP-sensitive channels were observed in isolated inside-out membrane patches from rat cultured central neurones. Two types of ATP-sensitive K+ channels were present in cortical neurones, one which had its open-state probability increased, the other its open-state probability decreased by application of ATP to the cytoplasmic membrane surface. Another, ATP-sensitive channel differing in ion conductance from all previously reported ATP-sensitive channels was also seen in patches from cortical neurones. This channel was noselective with respect to Na+, K+ and Cl ions and ATP produced a “flickery” type of block. The non-hydrolysable analogue, AMPPNP, did not mimic ATP and prevented ATP action Preliminary experiments indicate that similar, but not, identical ATP-sensitive channels exist in cerebellar neurones

Key words

Cultured central neuronesATP-sensitivePatch-clampIon channels

Copyright information

© Springer-Verlag 1988