Pflügers Archiv

, Volume 408, Issue 1, pp 32–37

Measurement by Quin2 of changes of the intracellular calcium concentration in strips of the rabbit ear artery and of the guinea-pig ileum

Authors

  • B. Himpens
    • Laboratorium voor FysiologieK.U.L.
  • R. Casteels
    • Laboratorium voor FysiologieK.U.L.
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology

DOI: 10.1007/BF00581837

Cite this article as:
Himpens, B. & Casteels, R. Pflugers Arch. (1987) 408: 32. doi:10.1007/BF00581837

Abstract

Ca2+ transient and force development were investigated in smooth muscle strips of the rabbit ear artery and the longitudinal layer of the guinea-pig ileum by using the fluorescent indicator Quin2. Agonists only transiently increased the flourescence intensity despite the enhanced contraction while excess potassium resulted in a maintained light signal. In Ca2+ free solutions the release by an agonist of Ca2+ from an intracellular store can be demonstrated. These observations illustrate the usefullness of the Ca2+ indicator Quin2 in the study of the excitation-contraction coupling in smooth muscle under various conditions.

Key words

Intracellular [Ca2+]Smooth muscleQuin2K+-depolarizationStimulation by agonists

Copyright information

© Springer-Verlag 1987