, Volume 411, Issue 1, pp 53-57

Calcium and secretagogues-induced conductances in rat exocrine pancreas

Purchase on Springer.com

$39.95 / €34.95 / £29.95*

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

The electrical properties of single acinar cells isolated from rat pancreas were studied with the whole-cell tight-seal recording method. Under resting conditions, the relative permeabilities of Cl and K wereP cl/P K≅3. At 1 μM internal calcium, a Ca and voltage-dependent Cl conductance was activated. At 10 μM internal calcium, the major conductance was selective for cations. It was not voltage-dependent. Acetylcholine and cholecystokinin induced an increase of internal Ca which in turn activated either only a Cl conductance or both Cl and cationic conductances. The secretagogue-induced conductance was increased to a variable extent by depolarisation. The absence of K channels activated by internal calcium indicates that, in pancreatic acinar cells, the mechanism of fluid secretion differs from that observed in other exocrine glands.