Pflügers Archiv

, Volume 359, Issue 1, pp 57–67

Effect on Mn2+ on permeability properties of frog skin

  • J. J. Hajjar
  • C. Abu-Murad
  • R. N. Khuri
  • R. Nassar
Article

DOI: 10.1007/BF00581277

Cite this article as:
Hajjar, J.J., Abu-Murad, C., Khuri, R.N. et al. Pflugers Arch. (1975) 359: 57. doi:10.1007/BF00581277

Summary

Mn2+ added to the inner bathing solution of frog skin caused a transient increase in potential difference (PD) and a decrease in total skin conductance and mannitol influx. Net Na flux and short-circuit current (Is. c.) were also reduced, the isotopic net flux being reduced more than Is. c. This observed discrepancy appears to be the result of Cl retention in the outer medium since it was not observed when the skin was bathed in a sulfate-substituted chloridefree solution. The effect of Mn2+ on the inner side of the frog skin appears to be due to a reduced permeation of Na+ and Cl through the outer barrier of the skin.

Addition of Mn2+ to the outer solution bathing the frog skin caused an increase in PD and a smaller increase in Is. c. These changes were not associated with alterations in the fluxes of Na+ or mannitol and were observed only when chloride was present in the bathing solutions. The effect of Mn2+ on this side of the frog skin may therefore be due to a net retention of Cl in the outer solution.

Key words

Effect of Manganese Ion Frog Skin Transmural Potential Difference Short-Circuit Current Sodium Fluxes 

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • J. J. Hajjar
    • 1
  • C. Abu-Murad
    • 1
  • R. N. Khuri
    • 1
  • R. Nassar
    • 1
  1. 1.Department of PhysiologyAmerican University of BeirutBeirutLebanon

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