Forelimb regeneration from different levels of amputation in the newt,Notophthalmus viridescens: Length, rate, and stages
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Some aspects of the influence of position on regeneration have been examined by comparing regeneration from two different levels along the newt forelimb.
We have defined a series of stages of forelimb regeneration in the newt,Notophthalmus viridescens, in order to facilitate this study.
Limbs amputated at either a proximal level (through the humerus) or a distal level (through the radius and ulna) pass through the same stages at the same times after amputation.
The histological sequence of events of digit regeneration was compared with that of limb regeneration from a proximal level of amputation and was found to be the same.
In limbs amputated at either proximal or distal levels, the rate of elongation of regenerates is the same during the phases of dedifferentiation, blastema accumulation, and blastema growth.
During the phase of differentiation and morphogenesis, the rate of elongation of regenerates from the proximal level is significantly greater than that of regenerates from the distal level.
The total length of regenerates from proximal and distal levels along the limb is significantly different.
The results indicate that positional information does not influence the developmental sequence of events of limb regeneration, but that it does influence the rate of growth of the regenerate and the specification of the structures to be replaced.
- Bryant, S. V., Bellairs, A. d'A.: Tail regeneration in the lizardsAnguis fragilis andLacerta dugesii. J. Linn. Soc. Zool.46, 297–305 (1967)
- Carlson, B. M.: The histology of inhibition of limb regeneration in the newt,Triturus, by actinomycin D. J. Morph.122, 249–264 (1967)
- Chalkley, D. T.: A quantitative histological analysis of forelimb regeneration inTriturus viridescens. J. Morph.94, 21–70 (1954)
- Ellis, M. M.: The relation of the amount of tail regenerated to the amount removed in tadpoles ofRana clamitans. J. exp. Zool.7, 421–455 (1909)
- Goodwin, P. A.: A comparison of regeneration rates and metamorphosis inTriturus andAmblystoma. Growth10, 75–87 (1946)
- Goss, R. J.: Principles of regeneration. New York: Academic Press 1969
- Liversage, R. A., Scadding, S. R.: Re-establishment of forelimb regeneration in adult hypophysectomizedDiemictylus (Triturus) viridescens given frog anterior pituitary extract. J. exp. Zool.170, 381–396 (1969)
- Maderson, P. F. A., Licht, P.: Factors influencing rates of tail regeneration in the lizardAnolis carolinensis. Experientia (Basel)24, 1083–1086 (1968)
- Maderson, P. F. A., Salthe, S. N.: Further observations on tail regeneration inAnolis carolinensis (Iguanidae, Lacertilia). J. exp. Zool.177, 185–189 (1971)
- Manner, H. W.: The origin of the blastema and of new tissues in regenerating forelimbs of adultTriturus viridescens virideseens (Rafinesque). J. exp. Zool.122, 229–257 (1953)
- Manner, H. W., Zapisek, W. F., Vallee, J. A.: The effect of body size on the rate of salamander limb regeneration. Anat. Rec.137, 378 (1960)
- Morgan, T. H.: The physiology of regeneration. J. exp. Zool.3, 457–500 (1906)
- Pritchett, W. H., Dent, J. N.: The role of size in the rate of limb regeneration in the adult newt. Growth36, 275–289 (1972)
- Rose, S. M.: Epidermal dedifferentiation during blastema formation in the regenerating limbs ofTriturus viridescens. J. exp. Zool.108, 337–361 (1948)
- Schauble, M. K.: Seasonal variation of newt forelimb regeneration under controlled environmental conditions. J. exp. Zool.181, 281–286 (1972)
- Singer, M.: The influence of the nerve in regeneration of the amphibian extremity. Quart. Rev. Biol.27, 169–200 (1952)
- Singer, M., Craven, L.: The growth and morphogenesis of the regenerating forelimb of the adultTriturus following denervation at various stages of development. J. exp. Zool.108, 279–308 (1948)
- Singer, M., Salpeter, M. M.: Regeneration in vertebrates: The role of the wound epithelium. In: Growth in living systems (Zarrow, M., ed.). New York: Basic Books 1961
- Schmidt, A. J.: Forelimb regeneration of thyroidectomized adult newts. I. Morphology. J. exp. Zool.137, 197–226 (1958a)
- Schmidt, A. J.: Forelimb regeneration of thyroidectomized adult newts. II. Histology. J. exp. Zool.139, 95–136 (1958b)
- Schmidt, A. J.: Molecular basis of regeneration: Enzymes. Illinois Monographs in Medical Sciences, vol. 6, No. 4. Urbana: Illinois Press 1966
- Schmidt, A. J.: Cellular biology of vertebrate regeneration and repair. Chicago: Chicago Univ. Press 1968
- Schotte, O. E., Butler, E. G.: Phases in regeneration of the urodele limb and their dependence upon the nervous system. J. exp. Zool.97, 95–121 (1944)
- Schotte, O. E., Hall, A. B.: Effect of hypophysectomy upon phases of regeneration in progress (Triturus viridescens). J. exp. Zool.121, 521–560 (1952)
- Spallanzani, A.: An essay on animal reproductions, 1768. (Translated from the Italian by M. Maty, London, 1769)
- Tassava, R. A., Goss, R. J.: Regeneration rate and amputation level in fish fins and lizard tails. Growth30, 9–21 (1966)
- Wolpert, L.: Positional information and pattern formation. In: Current topics in developmental biology, vol. 6 (Moscona, A. A., and Monroy, A., eds.), p. 183–224. New York: Academic Press 1971
- Zeleny, C.: Studies on the factors controlling the rate of regeneration. Illinois Biological Monographs, vol. 3, No. 1. Urbana: University of Illinois Press 1916
- Forelimb regeneration from different levels of amputation in the newt,Notophthalmus viridescens: Length, rate, and stages
Wilhelm Roux' Archiv für Entwicklungsmechanik der Organismen
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