Antonie van Leeuwenhoek

, Volume 60, Issue 2, pp 109–114

Degradation of galactomannan by aClostridium butyricum strain

  • Dong Xiuzhu
  • Philippe J. Y. M. J. Schyns
  • Alfons J. M. Stams
Article

DOI: 10.1007/BF00572700

Cite this article as:
Xiuzhu, D., Schyns, P.J.Y.M.J. & Stams, A.J.M. Antonie van Leeuwenhoek (1991) 60: 109. doi:10.1007/BF00572700

Abstract

An anaerobic, sporeforming, galactomannan-degrading bacterium was isolated from methanogenic granular sludge of an UASB reactor used for treatment of wastewater from a sugar refinery. The isolate degraded the polymer rapidly (doubling time was 55 min) and completely in mineral media. The bacterium was classified asClostridium butyricum; the main products were acetate, butyrate, hydrogen, formate, and presumably CO2. The strain produced extracellular endo-mannanase, cell-associated exo-mannanase and intracellular α-galactosidase activity. The mannanases were present when grown on galactomannan, but not after growth on glucose, galactose, or mannose.

Key words

anaerobic degradationClostridium butyricumfermentationgalactomannanhemicellulose

Abbreviations

PNPG

p-nitrophenyl-α-D-galactoside

PNPM

p-nitrophenyl-β-D-mannoside

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Dong Xiuzhu
    • 1
  • Philippe J. Y. M. J. Schyns
    • 1
  • Alfons J. M. Stams
    • 1
  1. 1.Department of MicrobiologyWageningen Agricultural UniversityWageningenThe Netherlands