Article

Virus Genes

, Volume 5, Issue 2, pp 147-156

First online:

An Epstein-Barr virus-producer line Akata: Establishment of the cell line and analysis of viral DNA

  • Kenzo TakadaAffiliated withDepartment of Microbiology, Nihon University School of Medicine
  • , Kenichi HorinouchiAffiliated withDepartment of Microbiology, Nihon University School of Medicine
  • , Yasushi OnoAffiliated withDepartment of Microbiology, Nihon University School of Medicine
  • , Takao AyaAffiliated withDepartment of Virology, Cancer Institute, Hokkaido University School of Medicine
  • , Toyoro OsatoAffiliated withDepartment of Virology, Cancer Institute, Hokkaido University School of Medicine
  • , Motoo TakahashiAffiliated withFirst Department of Surgery, Hokkaido University School of Medicine
  • , Shinichi HayasakaAffiliated withFirst Department of Surgery, Hokkaido University School of Medicine

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Abstract

An Epstein-Barr virus (EBV)-producer line, designated Akata, was established from a Japanese patient with Burkitt's lymphoma. The Akata line possessed the Burkitt's-type chromosome translocation, t(8q-; 14q+), and was derived from the tumor cell. Akata cells produced a large quantity of transforming virus upon treatment of cells with anti-immunoglobulin antibodies (Takada, 1984). Southern blot analysis of viral DNA indicated that the Akata EBV is nondefective and more representative of wild-type viruses. Akata cells should be useful as a source of EBV.

Key words

Epstein-Barr virus Burkitt's lymphoma B-cell line virus production viral DNA Southern analysis