Virus Genes

, Volume 5, Issue 2, pp 147–156

An Epstein-Barr virus-producer line Akata: Establishment of the cell line and analysis of viral DNA

Authors

  • Kenzo Takada
    • Department of MicrobiologyNihon University School of Medicine
  • Kenichi Horinouchi
    • Department of MicrobiologyNihon University School of Medicine
  • Yasushi Ono
    • Department of MicrobiologyNihon University School of Medicine
  • Takao Aya
    • Department of Virology, Cancer InstituteHokkaido University School of Medicine
  • Toyoro Osato
    • Department of Virology, Cancer InstituteHokkaido University School of Medicine
  • Motoo Takahashi
    • First Department of SurgeryHokkaido University School of Medicine
  • Shinichi Hayasaka
    • First Department of SurgeryHokkaido University School of Medicine
Article

DOI: 10.1007/BF00571929

Cite this article as:
Takada, K., Horinouchi, K., Ono, Y. et al. Virus Genes (1991) 5: 147. doi:10.1007/BF00571929

Abstract

An Epstein-Barr virus (EBV)-producer line, designated Akata, was established from a Japanese patient with Burkitt's lymphoma. The Akata line possessed the Burkitt's-type chromosome translocation, t(8q-; 14q+), and was derived from the tumor cell. Akata cells produced a large quantity of transforming virus upon treatment of cells with anti-immunoglobulin antibodies (Takada, 1984). Southern blot analysis of viral DNA indicated that the Akata EBV is nondefective and more representative of wild-type viruses. Akata cells should be useful as a source of EBV.

Key words

Epstein-Barr virusBurkitt's lymphomaB-cell linevirus productionviral DNASouthern analysis

Copyright information

© Kluwer Academic Publishers 1991