Virus Genes

, Volume 5, Issue 2, pp 147–156

An Epstein-Barr virus-producer line Akata: Establishment of the cell line and analysis of viral DNA

  • Kenzo Takada
  • Kenichi Horinouchi
  • Yasushi Ono
  • Takao Aya
  • Toyoro Osato
  • Motoo Takahashi
  • Shinichi Hayasaka
Article

DOI: 10.1007/BF00571929

Cite this article as:
Takada, K., Horinouchi, K., Ono, Y. et al. Virus Genes (1991) 5: 147. doi:10.1007/BF00571929

Abstract

An Epstein-Barr virus (EBV)-producer line, designated Akata, was established from a Japanese patient with Burkitt's lymphoma. The Akata line possessed the Burkitt's-type chromosome translocation, t(8q-; 14q+), and was derived from the tumor cell. Akata cells produced a large quantity of transforming virus upon treatment of cells with anti-immunoglobulin antibodies (Takada, 1984). Southern blot analysis of viral DNA indicated that the Akata EBV is nondefective and more representative of wild-type viruses. Akata cells should be useful as a source of EBV.

Key words

Epstein-Barr virusBurkitt's lymphomaB-cell linevirus productionviral DNASouthern analysis

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Kenzo Takada
    • 1
  • Kenichi Horinouchi
    • 1
  • Yasushi Ono
    • 1
  • Takao Aya
    • 2
  • Toyoro Osato
    • 2
  • Motoo Takahashi
    • 3
  • Shinichi Hayasaka
    • 3
  1. 1.Department of MicrobiologyNihon University School of MedicineTokyoJapan
  2. 2.Department of Virology, Cancer InstituteHokkaido University School of MedicineSapporoJapan
  3. 3.First Department of SurgeryHokkaido University School of MedicineSapporoJapan