Molecular Breeding

, Volume 2, Issue 3, pp 293–295

Vectors with rare-cutter restriction enzyme sites for expression of open reading frames in transgenic plants

  • Bärbel Überlacker
  • Wolfgang Werr
Short Communication

DOI: 10.1007/BF00564208

Cite this article as:
Überlacker, B. & Werr, W. Mol Breeding (1996) 2: 293. doi:10.1007/BF00564208

Abstract

Cloning of long open reading frames (ORFs) into plant gene expression vectors and transfer of the chimeric expression cassettes into binary vectors is often hampered by the presence of restriction enzyme cleavage sites internal to the open reading frame (ORF) to be expressed. We therefore modified the commonly used expression vector pRT100 [7] and several pGPTV binary vectors [2] by replacing 6 bp restriction sites with 8 bp sequences recognized by rare-cutter restriction enzymes.

Key words

plant gene expression vectors binary vectors large open reading frames 

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Bärbel Überlacker
    • 1
  • Wolfgang Werr
    • 1
  1. 1.Institut für Entwicklungsbiologie der Universität zu KölnKölnGermany

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