Biochemical Genetics

, Volume 32, Issue 3, pp 119–135

Drosophila melanogaster genes encoding three troponin-C isoforms and a calmodulin-related protein

  • Christine Fyrberg
  • Heather Parker
  • Bernadette Hutchison
  • Eric Fyrberg
Article

DOI: 10.1007/BF00554420

Cite this article as:
Fyrberg, C., Parker, H., Hutchison, B. et al. Biochem Genet (1994) 32: 119. doi:10.1007/BF00554420

Abstract

Using low-stringency hybridization and polymerase chain reaction (PCR)-based DNA amplification, we have isolated threeDrosophila melanogaster genes that encode troponin-C isoforms and one specifying a protein that is closely related to calmodulin. Two of the troponin-C genes, located within the 47D and 73F subdivisions of chromosomes 2 and 3, respectively, encode very closely related isoforms. That specified by the 47D gene accumulates almost exclusively in larval muscles, while that encoded by the 73F gene is present in both larvae and adults. The third gene, located within the 41C subdivision of chromosome 2, encodes a more distantly related troponin-C isoform that accumulates only within adults. The gene that encodes the calmodulin-related protein is located within the 97A subdivision of chromosome three. The protein encoded by this gene has a different primary sequence from that of conventional calmodulin, which is specified by a gene located within the 49A subdivision of chromosome 2. Our report is the first to describe insect troponin-C isoforms and further avails genetic methods for investigating thein vivo functions of the troponin-C/myosin light-chain/calmodulin protein superfamily.

Key words

calcium binding proteins muscle contraction muscle genes Drosophila genetics 

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Christine Fyrberg
    • 1
  • Heather Parker
    • 1
  • Bernadette Hutchison
    • 1
  • Eric Fyrberg
    • 1
  1. 1.Department of BiologyThe Johns Hopkins UniversityBaltimore

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