Parasitology Research

, Volume 73, Issue 5, pp 425–434

Identification and characterisation of proteins associated with the rhoptry organelles of Plasmodium falciparum merozoites

  • J. T. Clark
  • R. Anand
  • T. Akoglu
  • J. S. McBride
Original Investigations

DOI: 10.1007/BF00538200

Cite this article as:
Clark, J.T., Anand, R., Akoglu, T. et al. Parasitol Res (1987) 73: 425. doi:10.1007/BF00538200

Abstract

We describe antigens of Plasmodium falciparum recognised by murine monoclonal antibodies which by immunofluorescence react with the rhoptry organelles of the extracellular merozoite stage. Immunoblotting shows that the antibodies recognise two major parasite antigens of Mr 82 and 65 kilodaltons (kDa). Immunoprecipitations from detergent extracts of [35S]-methioninelabelled parasites show that the 82-kDa and 65-kDa antigens are parasite proteins. Pulse-chase experiments on synchronous parasite cultures show that the 82-kDa protein is synthesised during early schizogony and is later processed into the 65-kDa antigen in segmenting schizonts. In Nonidet P-40, these antigens are non-covalently associated with two other proteins of 40 kDa and 42 kDa. The 40/42-kDa doublet is synthesised in parallel with the 82 kDa antigen and persists, apparently unchanged, till the end of the cell cycle.

Abbreviations

HEPES

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

EDTA

ethylenediaminetetraacetic acid

EGTA

ethyleneglycolbis-(aminoethylether) tetraacetic acid

PMSF

phenylmethylsulphonylfluoride

TLCK

tosyl-L-lysine chloromethyl ketone

SDS-PAGE

sodium dodecyl sulphate polyacrylamide gel electrophoresis

Mr

relative molecular mass

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • J. T. Clark
    • 1
  • R. Anand
    • 1
  • T. Akoglu
    • 2
  • J. S. McBride
    • 1
  1. 1.Department of ZoologyUniversity of EdinburghEdinburghUK
  2. 2.Department of Internal MedicineCukurova UniversityAdanaTurkey

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