Abstract
The isoelectric characteristics of some nucleic acid preparations from rat liver have been examined. 10S and 4S RNA species and SV-DNA were found to have isoelectric points of 5.2, 6.0–6.7, and 4.35 respectively. The molecular charge ratios (net negative charge/nucleotide) were calculated. Using SV-DNA as a standard, these isoelectric characteristics and charge ratios have been interpreted as indicating that the 10S and 4S RNAs have 35 and 56% of the molecules involved in secondary structure.
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Sherbet, G.V., Lakshmi, M.S. & Cajone, F. Isoelectric characteristics and the secondary structure of some nucleic acids. Biophys. Struct. Mechanism 10, 121–128 (1983). https://doi.org/10.1007/BF00537554
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DOI: https://doi.org/10.1007/BF00537554