Article

Biophysics of structure and mechanism

, Volume 9, Issue 4, pp 235-244

The structure of bovine rhodopsin

  • P. A. HargraveAffiliated withDepartment of Medical Biochemistry, School of Medicine, Southern Illinois UniversityDepartment of Chemistry and Biochemistry, College of Science, Southern Illinois University
  • , J. H. McDowellAffiliated withDepartment of Medical Biochemistry, School of Medicine, Southern Illinois University
  • , Donna R. CurtisAffiliated withDepartment of Medical Biochemistry, School of Medicine, Southern Illinois University
  • , Janet K. WangAffiliated withDepartment of Medical Biochemistry, School of Medicine, Southern Illinois University
  • , Elizabeth JuszczakAffiliated withDepartment of Medical Biochemistry, School of Medicine, Southern Illinois University
  • , Shao-Ling FongAffiliated withDepartment of Medical Biochemistry, School of Medicine, Southern Illinois University
  • , J. K. Mohana RaoAffiliated withDepartment of Biological Sciences, Purdue University
  • , P. ArgosAffiliated withDepartment of Biological Sciences, Purdue University

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Abstract

We have isolated 16 peptides from a cyanogen bromide digest of rhodopsin. These cyanogen bromide peptides account for the complete composition of the protein. Methionine-containing peptides from other chemical and enzymatic digests of rhodopsin have allowed us to place the cyanogen bromide peptides in order, yielding the sequence of the protein. We have completed the sequence of most of the cyanogen bromide peptides. This information, in conjunction with that from other laboratories, forms the basis for our prediction of the secondary structure of the protein and how it may be arranged in the disk membrane.

Key words

Rhodopsin Amino acid sequence Secondary structure Topography Disk membrane