Archives of Dermatological Research

, Volume 277, Issue 6, pp 489–495

A noninvasive, in vivo technique to quantitatively measure water concentration of the stratum corneum using attenuated total-reflectance infrared spectroscopy

  • R. O. Potts
  • D. B. Guzek
  • R. R. Harris
  • J. E. McKie
Original Contributions

DOI: 10.1007/BF00510068

Cite this article as:
Potts, R.O., Guzek, D.B., Harris, R.R. et al. Arch Dermatol Res (1985) 277: 489. doi:10.1007/BF00510068

Summary

In order to noninvasively measure water concentration in the stratum corneum, infrared spectra were obtained using an attenuated total-reflectance technique in conjunction with Fourier transform spectroscopy. A weak water-absorbance band near 2,100 cm-1 was detected in both in vivo and in vitro spectra. The significance of this band is that it occurs in a region of the mid-infrared where the stratum corneum and most topically applied substances show no absorbance. In vitro spectra obtained as a function of ambient relative humidity showed an increase in the absorbance near 2,100 cm-1 with increasing water concentration in the stratum corneum. The combined in vivo and in vitro results lead to a quantitative assessment of water concentration in the uppermost layers of the stratum corneum.

Key words

Infrared spectraStratum corneumWater concentration

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • R. O. Potts
    • 1
  • D. B. Guzek
    • 1
  • R. R. Harris
    • 1
  • J. E. McKie
    • 1
  1. 1.Pfizer Central ResearchGrotonUSA