Biochemical Genetics

, Volume 19, Issue 3, pp 397–409

Effects of O2 stress on tomato alcohol dehydrogenase activity: Description of a second ADH coding gene

  • Steven D. Tanksley
  • Richard A. Jones

DOI: 10.1007/BF00504283

Cite this article as:
Tanksley, S.D. & Jones, R.A. Biochem Genet (1981) 19: 397. doi:10.1007/BF00504283


Subjecting tomato seedlings to anaerobic conditions results in expression of a previously undescribed Adh gene, Adh-2. Induction profiles were similar for all tissues, including roots, hypocotyls, cotyledons, and true leaves. In sharp contrast to ADH-1, ADH-2 showed no induction under anaerobic stress. The only time ADH-2 activity was expressed (under noninduced conditions) was during the early stages of embryogenesis. By late embryogenesis, ADH-2 activity approached a zero level, concomitant with a sharp rise in ADH-1 activity, which is found in the cotyledons of quiescent embryo. Despite striking differences in the regulation of these two genes, their homology is demonstrated in the ability of their enzyme subunits to form presumed intergenic heterodimers, which are visible during the transient period of embryogenesis when the polypeptides encoded by both genes are expressed. A multiple point linkage test using isozymic marker genes places the Adh-2 locus on chromosome 6 near Aps-1, whereas Adh-1 resides on chromosome 4.

Key words

Lycopersiconanaerobic inductionAdh-2linkage

Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • Steven D. Tanksley
    • 1
  • Richard A. Jones
    • 1
  1. 1.Department of Vegetable CropsUniversity of CaliforniaDavis
  2. 2.Department of HorticultureNew Mexico State UniversityLas Cruces