Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 281, Issue 1, pp 47–56

125I-labeled neurotoxin from clostridium botulinum A: Preparation, binding to synaptosomes and ascent to the spinal cord

  • E. Habermann
Article

DOI: 10.1007/BF00500611

Cite this article as:
Habermann, E. Naunyn-Schmiedeberg's Arch. Pharmacol. (1974) 281: 47. doi:10.1007/BF00500611

Summary

  1. 1.

    Labeling of crystalline botulinum A toxin has been done with 125I by aid of the chloramine T method. The neurotoxic component is well preserved, whereas the hemagglutinin undergoes physicochemical alterations. Neither with labeled nor with unlabeled toxin, hemagglutinating power parallels the main protein peak.

     
  2. 2.

    Neurotoxin, homogeneous in gel filtration, is bound to synaptosomes from rat brain. Cold toxin competes with labeled toxin, and antitoxin or neuraminidase partially remove the bound neurotoxin.

     
  3. 3.

    Upon intramuscular injection, some radioactivity is recovered in the respective parts of the spinal cord. Antitoxin prevents the ascent.

     

The similarities between tetanus and botulinum A neurotoxins are stressed.

Key words

Botulinum A Tetanus Neurotoxin Hemagglutinin Iodine Labeling 

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • E. Habermann
    • 1
  1. 1.Pharmakologisches Institut der Justus Liebig-UniversitätGießenGermany

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