Industrial Microbiology

European journal of applied microbiology and biotechnology

, Volume 9, Issue 1, pp 59-62

A method for turbidimetric measurement of bacterial growth in liquid cultures and agar plug diffusion cultures, using standard test tubes

  • K. J. BrownAffiliated withSection of Child Health Research, Department of Paediatrics, University of Auckland School of Medicine

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Summary

A method has been developed for turbidimetric measurement of bacterial growth in standard inexpensive test tubes with closures in-place. Liquid cultures and agar plug diffusion cultures can be assayed using an unmodified spectrophotometer. Growth curves of replicate cultures grown in test tubes, are reproducible with respect to similarity of curve shape, onset of logarithmic growth phase, and maximum growth.