, Volume 38, Issue 2, pp 173-180

A chlorine-free embedding medium for use in X-ray analytical electron microscope localisation of chloride in biological tissues

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A number of embedding media were checked for chlorine content by means of X-ray analytical electron microscopy (EMMA-4 electron microscope and KEVEX energy dispersive analyser). It was shown that the technique based on glutaraldehyde—urea polymerisation (Pease and Peterson, 1972) is satisfactory for use in ultrastructural detection of chloride ions by means of precipitation with silver ions.

Glutaraldehyde—urea does not contain chloride in detectable amounts and has the added advantage that dehydration and long periods of infiltration are not required.