Oxygen demand and supply in cell culture

  • Robert J. FleischakerJr.
  • Anthony J. Sinskey
Biotechnology

DOI: 10.1007/BF00499486

Cite this article as:
Fleischaker, R.J. & Sinskey, A.J. European J. Appl. Microbiol. Biotechnol. (1981) 12: 193. doi:10.1007/BF00499486

Summary

The oxygen demand of animal cells in microcarrier culture can be determined using a dissolved oxygen electrode. Continuous monitoring of the oxygen demand by human diploid fibroblasts shows it to be a good indicator of cell number. Furthermore, the oxygen demand of animal cells in large cultures can be supplied using membrane oxygenation using silicone rubber tubing. This method avoids the problems encountered with sparging the culture.

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Robert J. FleischakerJr.
    • 1
  • Anthony J. Sinskey
    • 1
  1. 1.Massachusetts Institute of TechnologyDepartment of Nutrition and Food ScienceCambridgeUSA