Rapid method for distinction of gram-negative from gram-positive bacteria
Received: 21 October 1977 DOI:
Cite this article as: Gregersen, T. European J. Appl. Microbiol. Biotechnol. (1978) 5: 123. doi:10.1007/BF00498806 Summary
A rapid method for distinction between gram-negative and grampositive bacteria by means of a 3% solution of potassium hydroxide is tested on 71 gram-positive and 55 gram-negative bacterial strains. The method proved reliable with one exception only, a
Bacillus macerans strain. That strain was definately gram-negative on staining. Other Bacillus strains were proved gram-positive by the test, even those being gram-negative on staining. References
Cerny, G. (1976). Method for the distinction of Gram-negative from Gram-positive bacteria. Eur. J. Appl. Microbiol.
Cowan, S. T. (1974). Cowan and Steel's Manual for the Identification of Medical Bacteria. 2nd ed. Cambridge: University Press
Cruickshank, R., Duguid, J.P., Marmion, B.P., Swain, R.H.A. (1973). Medical Microbiology. 12th ed. Vol. 1, p. 29. Churchill Livingstone
Davis, B.D., Dulbecco, R., Eisen, H., Ginsberg, H.S., Wood, W.B. (1967). Microbiology. p. 31. Harper International
Henriksen, S.D. (1976). Moraxella, Neisseria, Branhamella and Acinetobacter. Annu. Rev. Microbiol.
Migley, J.E.M. (1971) In: Methods in Microbiology, Morris and Ribbons, eds., Vol. 5A p. 334. Academic Press
Ryu, E. (1938). On the Gram-Differentiation of Bacteria by the simplest Method. J. Jpn. Soc. Vet. Sci.
17, p. 31
Ryu, E. (1940). A Simple Method of Differentiation between Gram-Positive and Gram-Negative Organisms without Staining. Kitasato Arch. Exp. Med.
Sakazaki, R. (1977). Personal communication
Stanier, R.Y., Adelberg, E.A., Ingraham, J.H. (1976). New Jersey. The Microbial World. 4th ed. Prentice Hall