, Volume 54, Issue 3, pp 237-250

Sudan Black B: Chemical structure and histochemistry of the blue main components

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Summary

Sudan Black B contains two blue main components, SSB-I and SSB-II. Their chemical structures were determinated by the aid of twodimensional thin-layer chromatography, column chromatography, absorption, IR, mass, H1-NMR, and C13-NMR spectroscopy and were proved by alternate synthesis. SSB-I has been found to be 2,3-dihydro-2,2-dimethyl-4-[(4-phenylazo-l-naphthalenyl)-azo]-lH-perimidine. For SSB-II was confirmed the known structure 2,3-dihydro-2,2-dimethyl-6-[(4-phenylazo-l-naphthalenyl)-azo]-lH-perimidine. Relations of chemical structure of SSB-I and SSB-II to their staining properties are discussed.

Professor Dr. G. Holle on the occasion on his 65th birthday