Histochemistry

, Volume 79, Issue 3, pp 405–415

Free radical signal of bile pigment in paraffin embedded liver tissue

Authors

  • G. Elek
    • Central Research Institute for Chemistry of the Hungarian Academy of Sciences
    • Department of Pathology of the Railway Hospital
  • A. Rockenbauer
    • Central Research Institute for Chemistry of the Hungarian Academy of Sciences
    • Department of Pathology of the Railway Hospital
  • L. Kovács
    • Central Research Institute for Chemistry of the Hungarian Academy of Sciences
    • Department of Pathology of the Railway Hospital
  • G. Bálint
    • Central Research Institute for Chemistry of the Hungarian Academy of Sciences
    • Department of Pathology of the Railway Hospital
Article

DOI: 10.1007/BF00491776

Cite this article as:
Elek, G., Rockenbauer, A., Kovács, L. et al. Histochemistry (1983) 79: 405. doi:10.1007/BF00491776

Summary

A linear correlation have been found between the amplitude of the free radical signal of the electron spin resonance (ESR) spectrum of paraffin embedded liver blocks and the number of bile casts in the histological sections made from these blocks. It has been suggested that the major part of the ESR free radical signal arises from bile pigment, but the contribution of “age pigment” cannot be excluded. On the basis of model experiments the majority of these radical centers was assigned to protein bound bilirubin. In the course of histological processing more than 80% of various free radical centers arising in air dried liver tissue is extracted.

Copyright information

© Springer-Verlag 1983