Biochemical Genetics

, Volume 8, Issue 3, pp 291–300

Abnormal stomatal behavior and root resistance, and hormonal imbalance in three wilty mutants of tomato

  • M. Tal
  • Y. Nevo

DOI: 10.1007/BF00486182

Cite this article as:
Tal, M. & Nevo, Y. Biochem Genet (1973) 8: 291. doi:10.1007/BF00486182


Three wilty mutants of tomato, flacca, sitiens, and notabilis, were compared with two control normal cultivars, Rheinlands Ruhm and Lukullus, for concentration of abscisic acid and root resistance to water flow. In addition, the three mutants treated with abscisic acid during development were compared with control mutant plants for stomatal opening and root resistance. The hormonal concentration was estimated by coleoptile assay and gas chromatography. Stomatal opening was estimated by measuring rate of transpiration and by examining leaf imprints. Root resistance was estimated by measuring the amount of exudate from roots of decapitated plants and the difference between the osmotic pressure of the exudate and the root medium. A lower level of abscisic acid was found in all three mutants as compared with the control normal plants. In addition, root resistance to water flow was higher in the three mutants than in the control normal types. All three mutants completely reverted to normal phenotypically, including stomatal and root resistances, when treated with abscisic acid. It has been suggested that the first hormonal change in the mutants is that of abscisic acid, and from it proceed the other changes.

Copyright information

© Plenum Publishing Corporation 1973

Authors and Affiliations

  • M. Tal
    • 1
  • Y. Nevo
    • 2
  1. 1.Division of Life SciencesThe Negev Institute for AridZone ResearchBeer ShevaIsrael
  2. 2.Department of BiologyThe Negev UniversityBeer ShevaIsrael