Biochemical Genetics

, Volume 14, Issue 11, pp 1041–1055

The enzymatic deficiency conditioned by the shrunken-1 mutations in maize

  • Prem S. Chourey
  • Oliver E. Nelson

DOI: 10.1007/BF00485135

Cite this article as:
Chourey, P.S. & Nelson, O.E. Biochem Genet (1976) 14: 1041. doi:10.1007/BF00485135


Evidence is presented to show that the Sh locus specifies sucrose synthetase in the developing endosperm of maize. The sh/sh/sh endosperm possesses less than 10% sucrose synthetase activity as compared to the normal Sh/Sh/Sh endosperm. The residual enzyme activity in five independently derived mutant genotypes is attributable to a protein molecule of different electrophoretic and immunochemical specificities that is presumably independent of the sh locus. Sucrose synthetase activity in the embryo in both the genotypes is electrophoretically indistinguishable from the one present in the mutant endosperm. Mutant endosperm has a reduced starch content as compared to the normal. This observation constitutes genetic evidence supporting a critical role for sucrose synthetase in starch biosynthesis.

Key words

sucrose synthetasetwo locistarch deficientshrunken mutantmaize

Copyright information

© Plenum Publishing Corporation 1976

Authors and Affiliations

  • Prem S. Chourey
    • 1
  • Oliver E. Nelson
    • 1
  1. 1.Department of GeneticsUniversity of WisconsinMadison