Biochemical Genetics

, Volume 16, Issue 7, pp 667–672

Simplified typing of mouse hemoglobin (Hbb) phenotypes using cystamine

  • J. Barry WhitneyIII

DOI: 10.1007/BF00484723

Cite this article as:
Whitney, J.B. Biochem Genet (1978) 16: 667. doi:10.1007/BF00484723


Cellulose acetate electrophoresis of mouse hemoglobins modified with the disulfide reagent cystamine permits rapid, unequivocal discrimination of all combinations of the codominant mouse hemoglobin “single” (Hbbs) and “diffuse” (Hbbd and Hbbp) alleles. The single, diffuse major, diffuse d-minor, and diffuse p-minor adult hemoglobins are all resolved by this method, which depends on the presence of a cysteine in the β chains of “diffuse” mice which is not found in the β chain of “single” mice.

Key words

hemoglobinelectrophoresisMus musculuspolymorphismalkylation

Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • J. Barry WhitneyIII
    • 1
  1. 1.The Jackson LaboratoryBar Harbor