, Volume 13, Issue 11-12, pp 759-769

Genetic control and intracellular localization of glutamate oxaloacetic transaminase in maize

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Abstract

Glutamate oxaloacetic transaminase (l-aspertate: 2-oxoglutarate aminotransferase, E.C. 2.6.1.1; GOT) was found to occur in five distinct electrophoretic forms in different tissue extracts from a number of highly inbred strains of Zea mays L. No major qualitative differences were detected in the various tissues examined, and the isozyme patterns did not undergo changes during temporal development of any given inbred strain. Cell fractionation studies showed one isozyme to be associated with the mitochondria (mGOT), another to be exclusively associated with the soluble fraction (sGOT), and a third to be associated with the glyoxysomes (gGOT). The glyoxysomal form occurs as two electrophoretically distinct variants which exist in different inbred strains of maize. The gGOT variants are under the control of two codiminant alleles (Got 1 A and Got 1 B ) at the Got 1 locus (isozyme5, gGOT). The genetic data and gene dosage effects suggest that GOT in maize is functionally a dimer.

This work was supported by U.S. Atomic Energy Commission Contract No. AT(38-1)-770.
A large portion of the experimental work was done in the Genetics Laboratory, University of South Carolina, where the authors were previously located.