Archives of Microbiology

, Volume 140, Issue 2, pp 101–106

Botryococcus braunii carbon/nitrogen metabolism as affected by ammonia addition

  • Masayuki Ohmori
  • Fred R. Wolf
  • James A. Bassham
Original Papers

DOI: 10.1007/BF00454910

Cite this article as:
Ohmori, M., Wolf, F.R. & Bassham, J.A. Arch. Microbiol. (1984) 140: 101. doi:10.1007/BF00454910

Abstract

Carbon metabolism in photosynthesizing and respiring cells of Botryococcus braunii was radically changed by the presence of 1 mM NH4Cl in the medium, when the so-called “resting state” previously had been subjected to a nitrogen-deficient medium. Ammonia addition to the algae photosynthesizing with 14C-labelled HCO3-almost completely inhibited the synthesis of 14C-labelled botryococcenes and other hexane-extractable compounds, and also inhibited the formation of insoluble compounds; however, it resulted in a large increase in the synthesis of alanine, glutamine, other amino acids, and especially of 5-aminolevulinic acid. Total CO2 fixation decreased about 60% and O2 evolution decreased more than 50%.

CO2 fixation in the dark with ammonia present led to labelled products derived from phosphoenolpyruvate carboxylation, such as glutamine, glutamate, and malate. Respiratory uptake of O2 increased by about 70%.

The inhibition of terpenoid synthesis and increased synthesis of C5 amino acids by Botryococcus upon ammonia addition indicates 1) a diversion of acetyl coenzyme A from synthetic pathways leading to terpenoids and 2) increased operation of pathways leading to the synthesis of amino acids, especially 5-aminolevulinic acid, a precursor to chlorophyll biosynthesis.

Key words

5-aminolevulinic acidAmmoniabotryococcus brauniiBotryococcenesCarbonHydrocarbonMetabolismNitrogenResting stateTerpenoids

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Masayuki Ohmori
    • 1
  • Fred R. Wolf
    • 1
  • James A. Bassham
    • 1
  1. 1.Laboratory of Chemical Biodynamics, Lawrence Berkeley LaboratoryUniversity of CaliforniaBerkeleyUSA
  2. 2.Ocean Research InstituteUniversity of TokyoTokyoJapan
  3. 3.Martin Marietta LaboratoriesBaltimoreUSA