Applied Microbiology and Biotechnology

, Volume 19, Issue 6, pp 422–426

Influence of pH and undissociated butyric acid on the production of acetone and butanol in batch cultures of Clostridium acetobutylicum

  • Fréderic Monot
  • Jean-Marc Engasser
  • Henri Petitdemange
Short Communication

DOI: 10.1007/BF00454381

Cite this article as:
Monot, F., Engasser, JM. & Petitdemange, H. Appl Microbiol Biotechnol (1984) 19: 422. doi:10.1007/BF00454381
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Summary

The kinetics of growth and acid and solvent production are examined in batch fermentation of Clostridium acetobutylicum at pH between 4.5 and 6.0. At the lower pH, growth occurs in two consecutive phases and solvents are the main excreted metabolites. At the higher pH, there is a single growth phase with only acid formation. The influence of the pH can be correlated with a critical role of the concentration of undissociated butyric acid in the medium: cellular growth is inhibited above 0.5 g/l and solvent production starts at an undissociated acid level of 1.5 g/l. Reducing the intracellular acid dissociation by lowering the intracellular pH also favours the production of acetone and butanol.

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Fréderic Monot
    • 1
  • Jean-Marc Engasser
    • 2
  • Henri Petitdemange
    • 2
  1. 1.Laboratoire des Sciences du Génie ChimiqueCNRS-ENSIC Institut National Polytechnique de LorraineNancy Cedex
  2. 2.Laboratoire de Chimie BiologiqueUniversité de NancyVandoeuvre les NancyFrance