Current Genetics

, Volume 9, Issue 8, pp 641–648

Mitochondrial suppression of a yeast nuclear mutation which affects the translation of the mitochondrial apocytochrome b transcript

  • Gerhard Rödel
  • Andreas Körte
  • Fritz Kaudewitz
Article

DOI: 10.1007/BF00449816

Cite this article as:
Rödel, G., Körte, A. & Kaudewitz, F. Curr Genet (1985) 9: 641. doi:10.1007/BF00449816

Summary

We describe a mitochondrial suppressor mutation, which restores respiratory competence to the nuclear pet-mutant MK2. This mutant lacks the message of the mitochondrialcob-gene and instead accumulates a partially spliced pre-mRNA which is not translated. Complete processing and translation of thecob-RNA is restored by a rearrangement of the mitochondrial DNA, leading to a fusion of thecob-coding sequences with the leader ofoli1, the mitochondrial gene coding for subunit IX of the ATPase. We conclude that the nuclear gene affected in MK2 is essential to allow translation of transcripts which contain thecob-leader sequence.

Key words

pet-mutantMitochondrial suppressioncob-leaderTranslation deficiency

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Gerhard Rödel
    • 1
  • Andreas Körte
    • 1
  • Fritz Kaudewitz
    • 1
  1. 1.Institut für Genetik und Mikrobiologie der Ludwig-Maximilians-UniversitätMünchen 19Germany