Original Papers

Archives of Microbiology

, Volume 152, Issue 5, pp 411-414

First online:

Influence of sugar source (lactose, glucose, galactose) on 2,3-butanediol production by Klebsiella oxytoca NRRL-B199

  • B. ChampluvierAffiliated withUnité de Chimie des Interfaces, Université Catholique de Louvain
  • , J. DecallonneAffiliated withUnité de Microbiologie, Université Catholique de Louvain
  • , P. G. RouxhetAffiliated withUnité de Chimie des Interfaces, Université Catholique de Louvain

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


The ability of Klebsiella oxytoca NRRL-B199 to use either lactose or the mixture of glucose and galactose as substrate for the production of 2,3-butanediol was studied in batch fermentations with different conditions of aeration and pH. 2,3-butanediol was undetected, or present in minute concentration in the fermentation broths with lactose, while it was the main product from glucose+galactose with final concentrations of up to 18.8 g/l in media at pH 6.0. Under conditions optimal for 2,3-butanediol synthesis, when aeration limited growth, the rate of biomass growth was more tightly related to the aeration rate in lactose medium than in glucose+galactose medium. These relations suggest that the growth rate is very low on lactose but still considerable on glucose+galactose when aeration rate tends toward zero. Correspondingly, the metabolism is more oxidative in the former medium, yielding mainly acetate as product.

Key words

2,3-Butanediol Lactose Glucose Galactose Klebsiella oxytoca NRRL-B199