Archives of Microbiology

, Volume 152, Issue 5, pp 411–414

Influence of sugar source (lactose, glucose, galactose) on 2,3-butanediol production by Klebsiella oxytoca NRRL-B199

  • B. Champluvier
  • J. Decallonne
  • P. G. Rouxhet
Original Papers

DOI: 10.1007/BF00446920

Cite this article as:
Champluvier, B., Decallonne, J. & Rouxhet, P.G. Arch. Microbiol. (1989) 152: 411. doi:10.1007/BF00446920


The ability of Klebsiella oxytoca NRRL-B199 to use either lactose or the mixture of glucose and galactose as substrate for the production of 2,3-butanediol was studied in batch fermentations with different conditions of aeration and pH. 2,3-butanediol was undetected, or present in minute concentration in the fermentation broths with lactose, while it was the main product from glucose+galactose with final concentrations of up to 18.8 g/l in media at pH 6.0. Under conditions optimal for 2,3-butanediol synthesis, when aeration limited growth, the rate of biomass growth was more tightly related to the aeration rate in lactose medium than in glucose+galactose medium. These relations suggest that the growth rate is very low on lactose but still considerable on glucose+galactose when aeration rate tends toward zero. Correspondingly, the metabolism is more oxidative in the former medium, yielding mainly acetate as product.

Key words

2,3-ButanediolLactoseGlucoseGalactoseKlebsiella oxytoca NRRL-B199



cell dry weight

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • B. Champluvier
    • 1
  • J. Decallonne
    • 2
  • P. G. Rouxhet
    • 1
  1. 1.Unité de Chimie des InterfacesUniversité Catholique de LouvainLouvain-la-NeuveBelgium
  2. 2.Unité de MicrobiologieUniversité Catholique de LouvainLouvain-la-NeuveBelgium