Archives of Microbiology

, Volume 125, Issue 3, pp 221-225

First online:

Lindane degradation by cell-free extracts of Clostridium rectum

  • N. OhisaAffiliated withDepartment of Agricultural Chemistry, University of Osaka Prefecture
  • , M. YamaguchiAffiliated withDepartment of Agricultural Chemistry, University of Osaka Prefecture
  • , N. KuriharaAffiliated withRadioisotope Research Center, Kyoto University

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For lindane degradation, a cell suspension of Clostridium rectum strain S-17 demands the addition of substrates such as leucine, alanine, pyruvate, a leucine-proline mixture, and molecular hydrogen. In the presence of leucine-proline mixture, lindane decomposed in parallel with isovaleric acid formation, and both lindane degradation and isovaleric acid formation were inhibited by monoiodoacetic acid, suggesting a close relation between lindane degradation and the Stickland reaction. Lindane was degraded by cell-free extracts of C. rectum in the presence of dithiothreitol (DTT). Radiogaschromatograms of n-hexane soluble metabolites from [14C] lindane showed the presence of monochlorobenzene and γ-3,4,5,6-tetrachlorocyclohexene, Leucine, NADH, and NADPH were somewhat less active than DTT for lindane degradation in cell-free extracts. Reductive dechlorination seemed the major route of lindane degradation in cell-free extracts as well as in the intact cells of C. rectum.

Key words

Clostridium rectum Lindane degradation Stickland reaction Monochlorobenzene Tetrachlorocyclohexene Reductive dechlorination