Lindane degradation by cell-free extracts of Clostridium rectum Article Received: 05 September 1979 DOI:
Cite this article as: Ohisa, N., Yamaguchi, M. & Kurihara, N. Arch. Microbiol. (1980) 125: 221. doi:10.1007/BF00446880 Abstract
For lindane degradation, a cell suspension of
Clostridium rectum strain S-17 demands the addition of substrates such as leucine, alanine, pyruvate, a leucine-proline mixture, and molecular hydrogen. In the presence of leucine-proline mixture, lindane decomposed in parallel with isovaleric acid formation, and both lindane degradation and isovaleric acid formation were inhibited by monoiodoacetic acid, suggesting a close relation between lindane degradation and the Stickland reaction. Lindane was degraded by cell-free extracts of C. rectum in the presence of dithiothreitol (DTT). Radiogaschromatograms of n-hexane soluble metabolites from [ 14C] lindane showed the presence of monochlorobenzene and γ-3,4,5,6-tetrachlorocyclohexene, Leucine, NADH, and NADPH were somewhat less active than DTT for lindane degradation in cell-free extracts. Reductive dechlorination seemed the major route of lindane degradation in cell-free extracts as well as in the intact cells of C. rectum. Key words Clostridium rectum Lindane degradation Stickland reaction Monochlorobenzene Tetrachlorocyclohexene Reductive dechlorination Abbreviations Lindane (γ-HCH)
Beland, F. A., Farwell, S. O., Robocker, A. E., Geer, R. D.: Electrochemical reduction and anaerobic degradation of lindane. J. Agric. Food Chem.
, 753–756 (1976)
Bradbury, F. R., Nield, P.: Amount of γ-benzene hexachloride picked up by resistant houseflies bred on a medium containing benzene hexachloride. Nature
, 1052–1053 (1953)
Chadwick, R. W., Chuang, L. T., Williams, K.: Dehydrogenation: a previously unreported pathway of lindane metabolism in mammals. Pestic. Biochem. Physiol.
, 575–586 (1975)
Fukami, H., Kimura, H., Nakazima, M.: On electrolysis of γ-BHC with the cathode controlled potential and determination of small amounts of benzene in aqueous alcohol solution. Botyu-Kagaku
, 51–57 (1953)
Heritage, A. D., MacRae, I. C.: Identification of intermediates formed during the degradation of hexachlorocyclohexanes by
. Appl. Environ. Microbiol.
, 1295–1297 (1977)
Jagnow, G., Haider, K., Ellwardt, P.: Anaerobic dechlorination and degradation of hexachlorocyclohexane isomers by anaerobic and facultative anaerobic bacteria. Arch. Microbiol.
, 285–292 (1977)
Kurihara, N., Nakajima, M.: Studies on BHC isomers and related compounds. VIII. Urinary metabolites produced from γ- and β-BHC in the mouse: chlorophenol conjugates. Pestic. Biochem. Physiol.
, 220–231 (1974)
Kurihara, N., Tanaka, K., Nakajima, M.: Anaerobic metabolism of lindane and related compounds by liver microsomes. In: Advances in pesticide science, Part 3 (H. Geissbühler, ed.), pp. 557–561. Oxford: Pergamon Press 1979
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.
, 265–275 (1951)
MacRae, I. C., Raghu, K., Bautista, E. M.: Anaerobic degradation of the insecticide lindane by
, 551–552 (1969)
Matsumura, F., Benezet, H. J., Patil, K. C.: Factors affecting microbial metabolism of γ-BHC. J. Pestic. Sci.
, 3–8 (1976)
Nisman, B.: The Stickland reaction. Bacteriol. Rev.
, 16–42 (1954)
Ohisa, N., Yamaguchi, M.: Gamma-BHC degradation accompanied by the growth of
isolated from paddy field soil. Agric. Biol. Chem.
, 1819–1823 (1978a)
Ohisa, N., Yamaguchi, M.: Degradation of gamma-BHC in flooded soil enriched with peptone. Agric. Biol. Chem.
, 1983–1987 (1978b)
Ohisa, N., Yamaguchi, M.:
species and γ-BHC degradation in paddy soil. Soil Biol. Biochem.
, 645–649 (1979)
Stadtman, T. C.: Studies on the enzymic reduction of amino acids. V. Coupling of a DPNH-generating system to glycine reduction. Arch. Biochem. Biophys.
, 36–44 (1962)
Tanaka, K., Kurihara, N., Nakajima, M.: Oxidative metabolism of lindane and its isomers with microsomes from rat liver and house fly abdomen. Pestic. Biochem. Physiol.
, 96–103 (1979)
Takeda, K., Furusaka, C.: Studies on the bacteria isolated anaerobically from paddy soil. III. Production of fatty acids and ammonia by
species. Soil Sci. Plant Nutr.
, 113–118 (1975)