Archives of Microbiology

, Volume 125, Issue 3, pp 221–225

Lindane degradation by cell-free extracts of Clostridium rectum

Authors

  • N. Ohisa
    • Department of Agricultural ChemistryUniversity of Osaka Prefecture
  • M. Yamaguchi
    • Department of Agricultural ChemistryUniversity of Osaka Prefecture
  • N. Kurihara
    • Radioisotope Research CenterKyoto University
Article

DOI: 10.1007/BF00446880

Cite this article as:
Ohisa, N., Yamaguchi, M. & Kurihara, N. Arch. Microbiol. (1980) 125: 221. doi:10.1007/BF00446880

Abstract

For lindane degradation, a cell suspension of Clostridium rectum strain S-17 demands the addition of substrates such as leucine, alanine, pyruvate, a leucine-proline mixture, and molecular hydrogen. In the presence of leucine-proline mixture, lindane decomposed in parallel with isovaleric acid formation, and both lindane degradation and isovaleric acid formation were inhibited by monoiodoacetic acid, suggesting a close relation between lindane degradation and the Stickland reaction. Lindane was degraded by cell-free extracts of C. rectum in the presence of dithiothreitol (DTT). Radiogaschromatograms of n-hexane soluble metabolites from [14C] lindane showed the presence of monochlorobenzene and γ-3,4,5,6-tetrachlorocyclohexene, Leucine, NADH, and NADPH were somewhat less active than DTT for lindane degradation in cell-free extracts. Reductive dechlorination seemed the major route of lindane degradation in cell-free extracts as well as in the intact cells of C. rectum.

Key words

Clostridium rectumLindane degradationStickland reactionMonochlorobenzeneTetrachlorocyclohexeneReductive dechlorination

Abbreviations

Lindane (γ-HCH)

γ-1,2,3,4,5,6-hexachlorocyclohexane

α-HCH

α-1,2,3,4,5,6-hexachlorocyclohexane

γ-TCH

γ-3,4,5,6-tetrachlorocyclohexene

γ-PCH

γ-1,3,4,5,6-pentachlorocyclohexene

DTT

1,4-dithiothreitol

Copyright information

© Springer-Verlag 1980