Archives of Microbiology

, Volume 123, Issue 2, pp 209–212

N-Glycyl-glucosamine: A novel constituent in the cell wall of Halococcus morrhuae

Authors

  • Josef Steber
    • Lehrstuhl für Mikrobiologie der Universität München
  • Karl Heinz Schleifer
    • Lehrstuhl für Mikrobiologie der Technischen Universität München
Article

DOI: 10.1007/BF00446822

Cite this article as:
Steber, J. & Schleifer, K.H. Arch. Microbiol. (1979) 123: 209. doi:10.1007/BF00446822

Abstract

The dinitrophenyl-derivative of N-glycylglucosamine was isolated from partially hydrolyzed dinitrophenylated cell walls of Halococcus morrhuae CCM 859. To increase the yield of amino-terminal glycine residues, halococcal cell walls were treated with alkali or acid prior to dinitrophenylation. Authentic N-glycyl-glucosamine was used as a reference substance. A substitution of the amino group of glucosamine by an amino acid has so far not been found in any other wall of a pro- or eucaryotic cell. Since only 5% of the glycine residues reveal an unsubstituted carboxyl group within intact cell walls, glycine may play a role in connecting glycan strands through peptidic linkages between the amino group of glucosamine and the carboxyl group of an uronic acid or gulosaminuronic acid.

Key words

N-Glycyl-glucosamineCell wallHatococcusArchaebacteria

Abbreviations

DNP

dinitrophenyl

Copyright information

© Springer-Verlag 1979