Archives of Microbiology

, Volume 149, Issue 6, pp 515–520

Base composition of DNA from symbiotic dinoflagellates: a tool for phylogenetic classification

  • Rudolf J. Blank
  • Volker A. R. Huss
  • Walter Kersten
Original Papers

DOI: 10.1007/BF00446754

Cite this article as:
Blank, R.J., Huss, V.A.R. & Kersten, W. Arch. Microbiol. (1988) 149: 515. doi:10.1007/BF00446754

Abstract

DNA of eight endosymbiotic dinoflagellates (zooxanthellae) from seven different host species has been analyzed as to its thermal characteristics and base composition by means of spectrophotometry and high performance liquid chromatography. All algae under investigation contain both methylcytosine and hydroxymethyluracil in addition to the bases typical of nuclear DNA. As a result, melting temperatures are decreased, suggesting lower contents of guanine plus cytosine than actually present. True percentages of guanine plus cytosine plus methylcytosine range from about 43 to 54 mol%. They are unique for the symbionts from different hosts, indicating phylogenetic separation of the taxa comparised within the genus Symbiodinium.

Key words

DinoflagellatesDNA compositionHydroxymethyluracilMethylcytosineSpeciationSymbiodiniumSymbiosisZooxanthellae

Abbreviations

dA

deoxyadenosine

dC

deoxycytidine

dG

deoxyguanosine

dT

deoxythymidine

m5dC

5-methyldeoxycytidine

hmdU

5-hydroxymethyldeoxyuridine

rC

ribocytidine

Br8G

bromine-80guanosine

A

adenine

C

cytosine

G

guanine

T

thymine

m5C

5-methylcytosine

hmU

5-hydroxymethyluracil

G+C

guanine plus cytosine plus 5-methylcytosine

HPLC

high performance liquid chromatography

Tm

temperature at the midpoint of hyperchromic shift

CTAB

N-cetyl-N,N,N-trimethyl-ammonium bromide

EDTA

ethylenediamine-tetraacetic acid, disodium salt

TRIS

tris-(hydroxymethyl)-aminomethane

1×SSC

standard saline citrate (0.15 M NaCl+0.015 M trisodium citrate, pH 7.0)

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Rudolf J. Blank
    • 1
  • Volker A. R. Huss
    • 1
  • Walter Kersten
    • 2
  1. 1.Institut für Botanik und Pharmazeutische BiologieUniversität Erlangen-NürnbergErlangenGermany
  2. 2.Institut für Biochemie der Medizinischen FakultätUniversität Erlangen-NürnbergErlangenGermany