Article

Archives of Microbiology

, Volume 111, Issue 1, pp 185-192

First online:

Some physiological observations on the uptake of d-glucose and 2-deoxy-d-glucose by starving and exponentially-growing yeasts

  • James A. BarnettAffiliated withSchool of Biological Sciences, University of East Anglia
  • , Anthony P. SimsAffiliated withSchool of Biological Sciences, University of East Anglia

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Abstract

Some methods for measuring the uptake of sugars by yeasts were investigated critically. A study was made of the effects of starvation of Pichia pinus, Candida utilis, Saccharomyces cerevisiae and Rhodosporidium toruloides on their uptake of d-glucose and 2-deoxy-d-glucose. Marked changes in the rates of uptake of these sugars occurred during 10 h of starvation, including (a) an immediate increase of up to 75% above that for growing cells and (b) a continuous decline to as little as 4%. Each yeast behaved differently. The rates did not remain constant during the periods of starvation often used for studies on the transport of sugars into yeasts. For Pichia pinus, there were striking differences, associated with starvation, between the transport of 2-deoxy-d-glucose and d-glucose, despite evidence that the two sugars enter this yeast by means of the same carrier. Some physiological explanations for these findings are discussed.

Key words

Yeasts Sugars d-Glucose 2-Deoxy-d-glucose Pichia pinus Transport Starvation Exponential growth Methodology Candida utilis Saccharomyces cerevisiae Rhodosporidium toruloides