Archives of Microbiology

, Volume 151, Issue 1, pp 54–58

Characterization of phosphonate uptake in two Phytophthora spp. and its inhibition by phosphate

Authors

  • T. Barchietto
    • Laboratoire de Pathologie VégétaleUniversité Pierre et Marie Curie
  • P. Saindrenan
    • Laboratoire de Pathologie VégétaleUniversité Pierre et Marie Curie
  • G. Bompeix
    • Laboratoire de Pathologie VégétaleUniversité Pierre et Marie Curie
Original Papers

DOI: 10.1007/BF00444669

Cite this article as:
Barchietto, T., Saindrenan, P. & Bompeix, G. Arch. Microbiol. (1988) 151: 54. doi:10.1007/BF00444669

Abstract

The uptake of the phosphonate ion, the active breakdown product in plant tissues of the systemic anti-Oomycete compound Fosetyl-Al (aluminium tris-Oethylphosphonate), was investigated in two Phytophthora spp. of differential sensitivity. Uptake was due to the simultaneous operation of two transport systems, one of low affinity (high K m) and one of high affinity (low K m). The relative contribution of each transport system varied with the external concentration of phosphonate, suggesting that phosphonate was a potent regulator of both systems. Phosphate was a partial competitive inhibitor with respect to phosphonate. Phosphate competed with phosphonate for uptake with a K i of 105 μM for P. cryptogea and 68 μM for P. citrophthora. Uptake was sensitive to pH, showing a maximum at pH 5.0 to 5.5. P. cryptogea was more efficient in phosphonate uptake, although it was less sensitive to inhibition by phosphonate in vitro, than P. citrophthora. This implied that the selective activity of phosphonate was not due to differential rates of uptake of this oxyanion. These results were discussed in relation to the mode of action of phosphonate towards Oomycetes.

Key words

Phosphonate Uptake Phosphate Inhibition Phytophthora cryptogea Phytophthora citrophthora

Copyright information

© Springer-Verlag 1989