Mycopathologia

, Volume 112, Issue 2, pp 105–112

Efficacy of brain heart infusion-egg albumen agar, yeast extract phosphate agar and peptone glucose agar media for isolation of Blastomyces dermatitidis from sputum

  • Sudha Chaturvedi
  • Harbans S. Randhawa
  • Vishnu P. Chaturvedi
  • Zia U. Khan
Article

DOI: 10.1007/BF00436505

Cite this article as:
Chaturvedi, S., Randhawa, H.S., Chaturvedi, V.P. et al. Mycopathologia (1990) 112: 105. doi:10.1007/BF00436505
  • 32 Downloads

Abstract

The efficacy of brain heart infusion (BHI)-egg albumen agar, yeast extract phosphate agar and several modified peptone glucose agar media was evaluated for isolation of Blastomyces dermatitidis from sputum concomitantly seeded with the yeast form of the pathogen and Candida albicans. Based upon high per cent culture positivity of sputum, improved recovery (CFU/ml) of the seeded inoculum, faster growth rate of B. dermatitidis and low level of contamination, BHI-egg albumen agar, followed by yeast extract phosphate agar are recommended as the media of choice for the isolation of B. dermatitidis from contaminated clinical specimens.

Key words

Blastomyces dermatitidis blastomycosis culture media isolation 

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Sudha Chaturvedi
    • 1
  • Harbans S. Randhawa
    • 1
  • Vishnu P. Chaturvedi
    • 1
  • Zia U. Khan
    • 1
  1. 1.Department of Medical Mycology, Vallabhbhai Patel Chest InstituteUniversity of DelhiDelhiIndia

Personalised recommendations