Efficacy of brain heart infusion-egg albumen agar, yeast extract phosphate agar and peptone glucose agar media for isolation of Blastomyces dermatitidis from sputum Article Received: 09 January 1990 Accepted: 05 February 1990 DOI:
Cite this article as: Chaturvedi, S., Randhawa, H.S., Chaturvedi, V.P. et al. Mycopathologia (1990) 112: 105. doi:10.1007/BF00436505 Abstract
The efficacy of brain heart infusion (BHI)-egg albumen agar, yeast extract phosphate agar and several modified peptone glucose agar media was evaluated for isolation of
Blastomyces dermatitidis from sputum concomitantly seeded with the yeast form of the pathogen and Candida albicans. Based upon high per cent culture positivity of sputum, improved recovery (CFU/ml) of the seeded inoculum, faster growth rate of B. dermatitidis and low level of contamination, BHI-egg albumen agar, followed by yeast extract phosphate agar are recommended as the media of choice for the isolation of B. dermatitidis from contaminated clinical specimens. Key words Blastomyces dermatitidis blastomycosis culture media isolation References
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