Current Genetics

, Volume 12, Issue 6, pp 413–420

Construction of brewer's yeasts secreting fungal endo-ß-glucanase

  • M. E. Penttilä
  • M. L. Suihko
  • U. Lehtinen
  • M. Nikkola
  • J. K. C. Knowles
Original Articles

DOI: 10.1007/BF00434818

Cite this article as:
Penttilä, M.E., Suihko, M.L., Lehtinen, U. et al. Curr Genet (1987) 12: 413. doi:10.1007/BF00434818
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Summary

Barley β-glucans present in wort reduce beer filtrability and cause hazes and precipitates in the finished beer. The endo-β-1,4-glucanase enzyme, EGI, found in the filamentous fungus Trichoderma reesei, is capable of efficiently hydrolyzing these β-glucans. The cDNA copy of the eg11 gene, which codes for the EGI enzyme, was coupled to yeast regulatory sequences and transferred to a brewer's yeast using the yeast copper chelatin gene CUP1 as a selection marker in the transformation. The eg11 gene was transferred to the yeast both on a multicopy plasmid and on an integrating plasmid. In both cases, highly glycosylated, active EGI enzyme was secreted into the medium. Barley β-glucans present in wort were efficiently hydrolyzed by the recombinant brewer's yeast.

Key words

Glucanolytic brewer's yeast Endo-β-1,4-glucanase Chromosomal integration Transformation Saccharomyces cerevisiae 

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. E. Penttilä
    • 1
  • M. L. Suihko
    • 1
  • U. Lehtinen
    • 1
  • M. Nikkola
    • 1
  • J. K. C. Knowles
    • 1
  1. 1.VTT Biotechnical LaboratoryEspooFinland

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