Current Genetics

, Volume 8, Issue 6, pp 413–421

Physical and gene mapping of cauliflower (Brassica oleracea) mitochondrial DNA

  • Philippe Chétritl
  • Chantal Mathieu
  • Jean Pierre Muller
  • Fernand Vedel
Original Articles

DOI: 10.1007/BF00433907

Cite this article as:
Chétritl, P., Mathieu, C., Muller, J.P. et al. Curr Genet (1984) 8: 413. doi:10.1007/BF00433907

Summary

A physical map of the mitochondrial DNA isolated from B. oleracea (cauliflower) inflorescences was constructed with the restriction endonucleases Sall, Kpnl and Bgll. Physical mapping was made using the multi enzyme method with either unlabeled or labeled DNA fragments isolated by preparative electrophoresis and a clone bank prepared by inserting incomplete Sall restriction digests of mitochondrial DNA into a cosmid vector.

The different mapping studies led to a circular map, about 217 kb in size, containing the entire sequence complexity of the genome. The 26S and 18S – 5S ribosomal RNA genes appeared to be separated by about 75 kb in this map. However, the particular cross-hybridization between several restriction fragments and the sequential diversity of some cosmids indicated that intra molecular recombination may occur naturally in higher plant mitochondria. Namely, one recombinational event resulted in the ribosomal RNA genes mapping closer together.

Key words

Mitochondrial DNARestriction and gene mappingCosmid bankRibosomal RNA

Abbreviations

mtDNA

mitochondrial DNA

kb

kilobasepairs

rRNA

ribosomal RNA

LGT agarose

low gelling temperature agarose

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Philippe Chétritl
    • 1
  • Chantal Mathieu
    • 1
  • Jean Pierre Muller
    • 2
  • Fernand Vedel
    • 1
  1. 1.Equipe de Biologic Cellulaire VégétaleLaboratoire de Cytophysiologie de la PhotosynthéseFrance
  2. 2.Centre de Génétique Moléculaire CNRSGif sur YvetteFrance