Marine Biology

, Volume 94, Issue 4, pp 625–630

Vibrio alginolyticus, a tetrodotoxin-producing bacterium, in the intestines of the fish Fugu vermicularis vermicularis

Authors

  • T. Noguchi
    • Laboratory of Marine Biochemistry, Faculty of AgricultureUniversity of Tokyo
  • D. F. Hwang
    • Laboratory of Marine Biochemistry, Faculty of AgricultureUniversity of Tokyo
  • O. Arakawa
    • Laboratory of Marine Biochemistry, Faculty of AgricultureUniversity of Tokyo
  • H. Sugita
    • Department of Fisheries, College of Agriculture and Veterinary MedicineNihon University
  • Y. Deguchi
    • Department of Fisheries, College of Agriculture and Veterinary MedicineNihon University
  • Y. Shida
    • Tokyo College of Pharmacy
  • K. Hashimoto
    • Laboratory of Marine Biochemistry, Faculty of AgricultureUniversity of Tokyo
Article

DOI: 10.1007/BF00431409

Cite this article as:
Noguchi, T., Hwang, D.F., Arakawa, O. et al. Mar. Biol. (1987) 94: 625. doi:10.1007/BF00431409

Abstract

To clarify the mechanism of toxification in animals contaminated with tetrodotoxin, the intestinal contents of the puffer Fugu vermicularis vermicularis were examined for bacterial flora in 1985. Twenty-six out of 33 strains belonged to the genus Vibrio. These bacteria were classified into Groups I to VII, based on biological and biochemical characters. High performance liquid chromatography and gas chromatography-mass spectrometry, together with mouse bioassay for toxicity, clearly demonstrated that Group I produced tetrodotoxin and anhydrotetrodotoxin under cultivation with a medium composed of Phytone peptone (BBL) and NaCl. Some other groups also produced this toxin and/or related substances to some extent. Strains of Group I were all identified as Vibrio alginolyticus. Two strains among four produced a detectable amount of tetrodotoxin and/or anhydrotetrodotoxin, as measured by all instrumental analyses applied. Our findings suggest that some strains of V. alginolyticus are closely related to the toxification of the puffer, and probably of other species.

Copyright information

© Springer-Verlag 1987