Antonie van Leeuwenhoek

, Volume 60, Issue 3, pp 145–158

Quantifying heterogeneity: flow cytometry of bacterial cultures

  • Douglas B. Kell
  • Hazel M. Ryder
  • Arseny S. Kaprelyants
  • Hans V. Westerhoff
Article

DOI: 10.1007/BF00430362

Cite this article as:
Kell, D.B., Ryder, H.M., Kaprelyants, A.S. et al. Antonie van Leeuwenhoek (1991) 60: 145. doi:10.1007/BF00430362

Abstract

Flow cytometry is a technique which permits the characterisation of individual cells in populations, in terms of distributions in their properties such as DNA content, protein content, viability, enzyme activities and so on. We review the technique, and some of its recent applications to microbiological problems. It is concluded that cellular heterogeneity, in both batch and continuous axenic cultures, is far greater than is normally assumed. This has important implications for the quantitative analysis of microbial processes.

Key words

analysisbacteriacytofluorometryflow cytometryheterogeneity

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Douglas B. Kell
    • 1
  • Hazel M. Ryder
    • 1
  • Arseny S. Kaprelyants
    • 2
  • Hans V. Westerhoff
    • 3
    • 4
  1. 1.Department of Biological SciencesUniversity College of WalesAberystwythUK
  2. 2.Bakh Institute of BiochemistryUSSR Academy of SciencesMoscowUSSR
  3. 3.Division of Molecular BiologyThe Netherlands Cancer InstituteAmsterdamThe Netherlands
  4. 4.E. C. Slater Institute for Biochemical ResearchUniversity of AmsterdamAmsterdamThe Netherlands