Original Papers

Archives of Microbiology

, Volume 148, Issue 1, pp 72-76

Orinithine as a constituent of the peptidoglycan of Chloroflexus aurantiacus, diaminopimelic acid in that of Chlorobium vibrioforme f. thiosulfatophilum

  • U. J. JürgensAffiliated withInstitut für Biologie II, Mikrobiologie, der Albert-Ludwigs-Universität
  • , J. MeißnerAffiliated withInstitut für Biologie II, Mikrobiologie, der Albert-Ludwigs-Universität
  • , U. FischerAffiliated withFachbereich Biologie, AG Geomikrobiologie der Universität Oldenburg
  • , W. A. KönigAffiliated withInstitut für Organische Chemie
  • , J. WeckesserAffiliated withInstitut für Biologie II, Mikrobiologie, der Albert-Ludwigs-Universität

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Abstract

L-Ornithine is the only diamino acid of the peptidoglycan of the gliding phototrophic Chloroflexus aurantiacus. The other constituents are L- and D-alanine, D-glutamic acid, N-acetyl-glucosamine and N-acetyl-muramic acid (in part as muramic acid-6-phosphate), all in approximate equimolar ratios to L-ornithine, aside from small amounts of glycine and histidine. Furthermore unlike typical Gram-negative bacteria, protein is not bound to this peptidoglycan. Instead, the rigid layer (sodium dodecyl sulfate insoluble cell wall fraction) contained large amounts of a complex polysaccharide consisting of sugar O-methyl ethers, hexoses and pentoses. Its binding site is presumably muramic acid-6-phosphate of the peptidoglycan.

In contrast, in Chlorobium vibrioforme f. thiosulfatophilium, meso-diaminopimelic acid was found as the only diamino acid of this peptidoglycan. As with other Gramnegative bacteria, L- and D-alanine, D-glutamic acid, N-acetyl-glucosamine and N-acetyl-muramic acid (no muramic acid-6-phosphate) were observed in approximate equimolar ratios to meso-diaminopimelic acid, except a lower D-alanine content. The rigid layer of Chlorobium vibrioforme f. thiosulfatophilum contained protein, and there were no indications for a complex polysaccharide comparable to that of Chloroflexus aurantiacus.

Key words

Chlorobium Chloroflexus Cell wall Diaminopimelic acid Muramic acid-6-phosphate Ornithine Peptidoglycan-polysaccharide complex