Archives of Microbiology

, Volume 148, Issue 1, pp 72–76

Orinithine as a constituent of the peptidoglycan of Chloroflexus aurantiacus, diaminopimelic acid in that of Chlorobium vibrioforme f. thiosulfatophilum

  • U. J. Jürgens
  • J. Meißner
  • U. Fischer
  • W. A. König
  • J. Weckesser
Original Papers

DOI: 10.1007/BF00429651

Cite this article as:
Jürgens, U.J., Meißner, J., Fischer, U. et al. Arch. Microbiol. (1987) 148: 72. doi:10.1007/BF00429651

Abstract

L-Ornithine is the only diamino acid of the peptidoglycan of the gliding phototrophic Chloroflexus aurantiacus. The other constituents are L- and D-alanine, D-glutamic acid, N-acetyl-glucosamine and N-acetyl-muramic acid (in part as muramic acid-6-phosphate), all in approximate equimolar ratios to L-ornithine, aside from small amounts of glycine and histidine. Furthermore unlike typical Gram-negative bacteria, protein is not bound to this peptidoglycan. Instead, the rigid layer (sodium dodecyl sulfate insoluble cell wall fraction) contained large amounts of a complex polysaccharide consisting of sugar O-methyl ethers, hexoses and pentoses. Its binding site is presumably muramic acid-6-phosphate of the peptidoglycan.

In contrast, in Chlorobium vibrioforme f. thiosulfatophilium, meso-diaminopimelic acid was found as the only diamino acid of this peptidoglycan. As with other Gramnegative bacteria, L- and D-alanine, D-glutamic acid, N-acetyl-glucosamine and N-acetyl-muramic acid (no muramic acid-6-phosphate) were observed in approximate equimolar ratios to meso-diaminopimelic acid, except a lower D-alanine content. The rigid layer of Chlorobium vibrioforme f. thiosulfatophilum contained protein, and there were no indications for a complex polysaccharide comparable to that of Chloroflexus aurantiacus.

Key words

ChlorobiumChloroflexusCell wallDiaminopimelic acidMuramic acid-6-phosphateOrnithinePeptidoglycan-polysaccharide complex

Abbreviations

Ala

alanine

A2pm

diaminopimelic acid

GC/MS

combined gas-liquid chromatography/mass spectrometry

GlcNAc

N-acetyl-glucosamine

Glu

glutamic acid

Gly

glycine

HF

hydrofluoric acid

Lys

lysine

MurNAc

N-acetyl-muramic acid

Orn

ornithine

SDS

sodium dodecyl sulfate

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • U. J. Jürgens
    • 1
  • J. Meißner
    • 1
  • U. Fischer
    • 2
  • W. A. König
    • 3
  • J. Weckesser
    • 1
  1. 1.Institut für Biologie II, Mikrobiologieder Albert-Ludwigs-UniversitätFreiburg i. Br.Federal Republic of Germany
  2. 2.Fachbereich BiologieAG Geomikrobiologie der Universität OldenburgOldenburgFederal Republic of Germany
  3. 3.Institut für Organische ChemieHamburg 13Federal Republic of Germany