Archives of Microbiology

, Volume 108, Issue 2, pp 189–194

Synchronized cultures of a cell wall-less mutant of Chlamydomonas reinhardii

Authors

  • Torleiv Lien
    • Institute for General MicrobiologyUniversity of Bergen
    • Botanical LaboratoryUniversity of Bergen
  • Gjert Knutsen
    • Institute for General MicrobiologyUniversity of Bergen
    • Botanical LaboratoryUniversity of Bergen
Article

DOI: 10.1007/BF00428950

Cite this article as:
Lien, T. & Knutsen, G. Arch. Microbiol. (1976) 108: 189. doi:10.1007/BF00428950

Abstract

Synchronization and synchronous growth of a cell wall-less mutant of Chlamydomonas reinhardii have been described. The following growth conditions were used: A modified Sueokas' “high salt minimal medium”, 14∶10 h light-dark cycle, growth temperature 30°C, light intensity 12–18 Klux and dilution of the culture at the end of the dark to a constant cell density of 1.0·106 cells/ml. The time course of increase and distribution of cell volume, cytoplasmic and nuclear division, release of motile cells after the division period and accumulation of DNA, RNA and protein are reported. These mutant cells did not make any sporangium in which the dividing cells were kept as a unit inside a mother cell wall. However, they usually adhered during the period of division, thus making clumps containing 2, 4 and 8 cells. Several of these cell clumps dissolved releasing either single or couples of 2 and 4 cells. After the end of division the cells became flagellated and motile and thereby releasing themselves from the aggregate.

Key words

Chlamydomonas reinhardiiSynchronous growthCell wall-less mutant

Non-Standard Abbreviations

AWV

average weighed cell volume

MM

minimal medium

HSM

high salt medium

TCA

trichloroacetic acid

Copyright information

© Springer-Verlag 1976