, Volume 127, Issue 2, pp 167-169

Analysis of the fermentation pathways of clostridia using double labelled glutamade

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Abstract

l-(4-14C, 3-3H)Glutamate was used as a tool to elucidate the pathway of its fermentation. The methylaspartate pathway was found in strains of Clostridium tetanomorphum, C. malenominatum, C. limosum, C. lentoputrescens and C. tetani, whereas the hydroxyglutarate pathway was detected in C. sporosphaeroides. These data were supported by measurement of enzyme activities present in cell-free extracts.