Archives of Microbiology

, Volume 127, Issue 2, pp 167–169

Analysis of the fermentation pathways of clostridia using double labelled glutamade

  • Wolfgang Buckel
Short Communications

DOI: 10.1007/BF00428021

Cite this article as:
Buckel, W. Arch. Microbiol. (1980) 127: 167. doi:10.1007/BF00428021


l-(4-14C, 3-3H)Glutamate was used as a tool to elucidate the pathway of its fermentation. The methylaspartate pathway was found in strains of Clostridium tetanomorphum, C. malenominatum, C. limosum, C. lentoputrescens and C. tetani, whereas the hydroxyglutarate pathway was detected in C. sporosphaeroides. These data were supported by measurement of enzyme activities present in cell-free extracts.

Key words

Clostridial-4-14C, 3-3H)GlutamateButyraten-PropylamineMethylaspartate and hydroxyglutarate pathways

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Wolfgang Buckel
    • 1
  1. 1.Biochemie IFakultät für Biologie und Vorklinische Medizin der Universität RegensburgRegensburgFederal Republic of Germny