Molecular and General Genetics MGG

, Volume 215, Issue 3, pp 490–500

Dependence of inessential late gene expression on early meiotic events in Saccharomyces cerevisiae

  • Gautam Kao
  • Daniel G. Mannix
  • Brian L. Holaway
  • Mary C. Finn
  • Andrea E. Bonny
  • Mary J. Clancy
Article

DOI: 10.1007/BF00427048

Cite this article as:
Kao, G., Mannix, D.G., Holaway, B.L. et al. Molec. Gen. Genet. (1989) 215: 490. doi:10.1007/BF00427048
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Summary

SPR3 is one of at least nine genes which are expressed in sporulating Saccharomyces cerevisiae cells at the time of meiosis I. We show below that strains homozygous for null alleles of SPR3 are capable of normal meiosis and the production of viable ascospores. We have also monitored SPR3 expression in a series of strains that are defective in meiotic development, using an SPR3: lacZ fusion carried on a single copy plasmid. β-Galactosidase activity occurred at wild-type levels in diploid strains homozygous for mutations in spo13, rad50, rad57 and cdc9, but was greatly reduced in strains carrying cdc8 or spo7 defects. We conclude that SPR3 expression is a valid monitor of early meiotic development, even though the gene is inessential for the sporulation process.

Key words

MeiosisSaccharomyces cerevisiaeSporulationInessential genesMeiosis

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Gautam Kao
    • 1
  • Daniel G. Mannix
    • 1
  • Brian L. Holaway
    • 1
  • Mary C. Finn
    • 1
  • Andrea E. Bonny
    • 1
  • Mary J. Clancy
    • 1
  1. 1.Department of Biological SciencesUniversity of Notre DameNotre DameUSA
  2. 2.Botany DepartmentUniversity of GeorgiaAthensUSA