Archives of Dermatological Research

, Volume 281, Issue 2, pp 105–110

Biochemical and biological profile of a new enzyme preparation from antarctic krill (E. superba) suitable for debridement of ulcerative lesions

Authors

  • J. -E. Anheller
    • Pharmacia AB
  • L. Hellgren
    • Department of DermatologyUniversity of Umeå
  • B. Karlstam
    • Pharmacia AB
  • J. Vincent
    • Department of DermatologyUniversity of Umeå
Original Contributions

DOI: 10.1007/BF00426587

Cite this article as:
Anheller, J.-., Hellgren, L., Karlstam, B. et al. Arch Dermatol Res (1989) 281: 105. doi:10.1007/BF00426587

Summary

A protease extract from Antarctic krill (E. superba) intended as a new enzymatic debrider for necrotic ulcers has been characterized by sodium dodecyl sulphate polyacrylamide gradient gel electrophoresis and fast protein liquid chromatography. The predominant enzymes in the preparation represent trypsin-like activity associated with three serine proteinases. In addition two carboxypeptidases A and B are present as cooperative enzymes for a more complete breakdown of complex proteinaceous substrates. Biological studies on a well-defined substrate (fibrin) originating from leg ulcers, demonstrated more effective degradation by krill enzymes than bovine trypsin, a common component in marketed enzymatic debriders. These findings support previously in vitro/in vivo studies in an animal model (rat) using excised rat skin as “necrotic” tissue.

Key words

Antarctic krill (E. superba)ProteasesBiochemical/biological profileEnzymatic debridement

Abbreviations

TAME

p-toluene-sulfonyl-l-arginine methyl ester; hipp-l-phe hippuryl-l-phenylalanine; hipp-l-arg, hippuryl-l-arginine

Cpase

carboxypeptidase

SDS

sodium dodecyl sulphate

Copyright information

© Springer-Verlag 1989