Molecular and General Genetics MGG

, Volume 201, Issue 2, pp 360–362

Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli

  • Yasunobu Kano
  • Setsuo Yoshino
  • Morimasa Wada
  • Kazushige Yokoyama
  • Masahiro Nobuhara
  • Fumio Imamoto
Short Communication

DOI: 10.1007/BF00425687

Cite this article as:
Kano, Y., Yoshino, S., Wada, M. et al. Molec. Gen. Genet. (1985) 201: 360. doi:10.1007/BF00425687

Summary

The Escherichia coli HU-1 was cloned by use of mixed synthetic oligonucleotides (17-mer) predicted from a portion of its amino acid sequence. The amino acid sequence of the HU-1 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream from the translational initiation codon (GUG) of the HU-1 gene.

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Yasunobu Kano
    • 1
  • Setsuo Yoshino
    • 2
  • Morimasa Wada
    • 1
  • Kazushige Yokoyama
    • 1
  • Masahiro Nobuhara
    • 3
  • Fumio Imamoto
    • 1
  1. 1.Laboratory of Molecular Genetics(Riken) The Institute of Physical and Chemical ResearchWako, Saitama
  2. 2.Central Research LaboratoriesMitsui Toatsu Chemicals, Inc.Yokohama, Kanagawa
  3. 3.Research Laboratories of Cell ScienceMochida Pharmaceutical Co., LTDTokyoJapan