, Volume 201, Issue 2, pp 347-350

Amplification of the ArgF region in strain HfrP4X of E. coli K-12

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In E. coli K-12 the argF gene is flanked by ISI sequences in direct repeat. Mutants that overproduce the argF-coded enzyme ornithine transcarbamylase can be selected; we have shown that in one class of these mutants there is an approximately forty five-fold amplification of the region bounded by the ISI repeats. This class of mutants has been detected only in strains in which the F-factor is integrated in cis to the region.

Communicated by P. Starlinger