Archives of Microbiology

, Volume 136, Issue 4, pp 254–261

Methanococcus jannaschii sp. nov., an extremely thermophilic methanogen from a submarine hydrothermal vent

  • W. J. Jones
  • J. A. Leigh
  • F. Mayer
  • C. R. Woese
  • R. S. Wolfe
Original Papers

DOI: 10.1007/BF00425213

Cite this article as:
Jones, W.J., Leigh, J.A., Mayer, F. et al. Arch. Microbiol. (1983) 136: 254. doi:10.1007/BF00425213

Abstract

A new extremely thermophilic methane-producing bacterium was isolated from a submarine hydrothermal vent sample collected by a research team from the Woods Hole Oceanographic Institution using the manned submersible ALVIN. The sample was obtained from the base of a “white smoker” chimney on the East Pacific Rise at 20° 50′ N latitude and 109° 06′ W longitude at a depth of 2600 m. The isolate was a motile irregular coccus with an osmotically fragile cell wall and a complex flagellar system. In defined medium with 80% H2 and 20% CO2, the isolate had a doubling time of 26 min at 85° C. The pH range for growth was 5.2 to 7.0 with an optimum near 6.0. NaCl was required for growth with an optimum of 2 to 3% (w/v). The mol % G+C was 31%. In cell-free extracts, methane formation from methylcoenzyme M was temperature-dependent, and H2 or formate served as electron donors. Methane formation from H2 and CO2 occurred at a much lower rate. Oligonucleotide cataloging of the 16S ribosomal RNA established the isolate as a new species of the genus Methanococcus and the name Methanococcus jannaschii is proposed. The isolation of M. jannaschii from a submarine hydrothermal vent provides additional evidence for biogenic production of CH4 from these deep-sea environments.

Key words

Methanococcus jannaschiiThermophilic methanogenic bacteriaMethanococcusHydrothermal vent methanogenDeep sea methanogen

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • W. J. Jones
    • 1
  • J. A. Leigh
    • 1
  • F. Mayer
    • 2
  • C. R. Woese
    • 3
  • R. S. Wolfe
    • 1
  1. 1.Department of MicrobiologyUniversity of IllinoisUrbanaUSA
  2. 2.Institut für Mikrobiologie der Universität GöttingenGöttingenGermany
  3. 3.Department of Genetics and DevelopmentUniversity of IllinoisUrbanaUSA