Molecular and General Genetics MGG

, Volume 203, Issue 3, pp 430–434

Heterologous expression and regulation of the lysA genes of Pseudomonas aeruginosa and Escherichia coli

  • Christine Martin
  • Brigitte Cami
  • Françoise Borne
  • Davis J. Jeenes
  • Dieter Haas
  • Jean-Claude Patte
Article

DOI: 10.1007/BF00422067

Cite this article as:
Martin, C., Cami, B., Borne, F. et al. Mol Gen Genet (1986) 203: 430. doi:10.1007/BF00422067

Summary

The Pseudomonas aeruginosa lysA gene encoding diaminopimelate decarboxylase (DAP-decarboxylase) was cloned into a broad host range vector. This gene complemented a lys mutation at the lys-12 locus of P. aeruginosa and a lysA defect in Escherichia coli. The P. aeruginosa DAP-decarboxylase was synthesized constitutively in P. aeruginosa as well as in E. coli, where the Pseudomonas lysA gene was poorly expressed. By contrast, the E. coli lysA gene was expressed well in P. aeruginosa and subject to lysine regulation when the E. coli LysR activator protein was provided. This indicates that the mechanism of transcriptional activation for the E. coli lysA gene is effective in the heterologous host.

Key words

PseudomonaslysAHeterologous expression

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Christine Martin
    • 1
  • Brigitte Cami
    • 1
  • Françoise Borne
    • 1
  • Davis J. Jeenes
    • 2
  • Dieter Haas
    • 2
  • Jean-Claude Patte
    • 1
  1. 1.Laboratoire de Chimie BactérienneCentre National de la Recherche ScientifiqueMarseille Cedex 9France
  2. 2.Mikrobiologisches InsitutEidgenossische Technische Hochschule ZurichZurichSwitzerland